Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex

Nat Struct Mol Biol. 2009 Jul;16(7):782-8. doi: 10.1038/nsmb.1618. Epub 2009 Jun 7.


The nuclear pore complex mediates nucleocytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, collectively known as nucleoporins. Nucleoporins are organized into distinct subcomplexes. We optimized the isolation of a putative membrane-coating subcomplex of the nuclear pore complex, the heptameric Nup84 complex, and analyzed its structure by EM. Our data confirmed the previously reported 'Y' shape. We discerned additional structural details, including specific hinge regions at which the particle shows great flexibility. We determined the three-dimensional structures of two conformers, mapped the localization of two nucleoporins within the subcomplex and docked known crystal structures into the EM maps. The free ends of the Y-shaped particle are formed by beta-propellers; the connecting segments consist of alpha-solenoids. Notably, the same organizational principle is found in the clathrin triskelion, which may share a common evolutionary origin with the heptameric complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray
  • Humans
  • Models, Molecular
  • Nuclear Envelope / chemistry*
  • Nuclear Pore / chemistry*
  • Nuclear Pore / metabolism
  • Nuclear Pore Complex Proteins / chemistry
  • Nuclear Pore Complex Proteins / isolation & purification
  • Nuclear Pore Complex Proteins / metabolism
  • Nuclear Pore Complex Proteins / ultrastructure*
  • Protein Conformation*
  • Protein Multimerization
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / isolation & purification
  • Saccharomyces cerevisiae Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / ultrastructure*


  • NUP84 protein, S cerevisiae
  • Nuclear Pore Complex Proteins
  • Saccharomyces cerevisiae Proteins