Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart

J Biophotonics. 2009 Jul;2(6-7):380-8. doi: 10.1002/jbio.200910021.

Abstract

This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster: optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross-sectional OCT and C-scan LSFM images has been developed. With this dual-imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila melanogaster / anatomy & histology
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / physiology*
  • Female
  • Fourier Analysis
  • Gene Expression Regulation
  • Heart / physiology*
  • Larva / physiology
  • Microfilament Proteins / metabolism
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Protein Transport
  • Systems Integration
  • Tomography, Optical Coherence / instrumentation
  • Tomography, Optical Coherence / methods*

Substances

  • Microfilament Proteins
  • moesin