Non-Hodgkin lymphoma (NHL) subtypes defined by common translocations: utility of fluorescence in situ hybridization (FISH) in a case-control study

Leuk Res. 2010 Feb;34(2):190-5. doi: 10.1016/j.leukres.2009.05.007. Epub 2009 Jun 7.


We used fluorescence in situ hybridization (FISH) assays to identify t(14;18) translocations in archival paraffin-embedded tumor sections from non-Hodgkin lymphoma (NHL) cases enrolled in a population-based study. t(14;18) was identified in 54% of 152 cases, including 39% of diffuse large cell lymphomas (26 of 66 cases) and 84% of follicular lymphomas (36 of 43 cases). Eighty-seven percent of t(14;18)-positive cases and 57% of t(14;18)-negative cases expressed bcl-2. FISH assays detected twice as many t(14;18)-positive follicular lymphomas as PCR assays. Overall, study findings support the use of FISH assays to detect t(14;18) in archival tumor samples for epidemiologic studies of NHL subtypes.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Chromosomes, Human, Pair 14
  • Chromosomes, Human, Pair 18
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • In Situ Hybridization, Fluorescence / standards
  • Lymphoma, Follicular / genetics*
  • Lymphoma, Large B-Cell, Diffuse / genetics*
  • Lymphoma, Non-Hodgkin / classification*
  • Lymphoma, Non-Hodgkin / genetics*
  • Proto-Oncogene Proteins c-bcl-2 / analysis
  • Translocation, Genetic*


  • Proto-Oncogene Proteins c-bcl-2