MicroRNA-1 regulates cardiomyocyte apoptosis by targeting Bcl-2

Int Heart J. 2009 May;50(3):377-87. doi: 10.1536/ihj.50.377.


MicroRNA-1 (miR-1) is preferentially expressed in cardiac muscles, and the expression has been demonstrated to be involved in cardiac development and cardiovascular diseases. Here we report that miR-1 is closely related with ischemia/reperfusion injury in a rat model. The level of miR-1 is inversely correlated with Bcl-2 protein expression in cardiomyocytes of the I/R rat model. In vitro, the level of miR-1 was dramatically increased in response to H(2)O(2). Overexpression of miR-1 facilitated H(2)O(2)-induced apoptosis in cardiomyocytes. Inhibition of miR-1 by antisense inhibitory oligonucleotides caused marked resistance to H(2)O(2). Through bioinformatics, we identified the potential target sites for miR-1 on the 3' UTR of Bcl-2. miR-1 significantly reduced the expression of Bcl-2 in the levels of mRNA and protein. The post-transcriptional repression of Bcl-2 was further confirmed by luciferase reporter experiments. These data demonstrated that miR-1 plays an important role in the regulation of cardiomyocyte apoptosis, which is involved in post-transcriptional repression of Bcl-2.

MeSH terms

  • Animals
  • Apoptosis / genetics*
  • Cell Line
  • Gene Expression Regulation / genetics
  • MicroRNAs / genetics
  • Myocytes, Cardiac / metabolism*
  • Protein Processing, Post-Translational / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction


  • MIRN1 microRNA, rat
  • MicroRNAs
  • Proto-Oncogene Proteins c-bcl-2