miR-29b expression is associated with disease-free survival in patients with ovarian serous carcinoma

Int J Gynecol Cancer. 2009 May;19(4):641-7. doi: 10.1111/IGC.0b013e3181a48cf9.


Micro-RNAs are a group of small noncoding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature micro-RNAs in human cancers. We characterized the alteration in expression of miR-29b in ovarian serous carcinoma. miR-29b expression was analyzed using quantitative stem-loop reverse transcriptase polymerase chain reaction on a set of 50 formalin-fixed, paraffin-embedded ovarian serous carcinoma samples. Protein expression of p53, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, Ki-67, and insulinlike growth factor 1 was quantified in the corresponding tissue microarray. The expression profile of miR-29b was correlated with clinicopathological and patient survival data. We provide definitive evidence that miR-29b is down-regulated in a significant proportion of ovarian serous carcinomas and is associated with specific clinicopathological features, most notably high miR-29b expression being associated with reduced disease-free survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cystadenocarcinoma, Serous / genetics*
  • Cystadenocarcinoma, Serous / metabolism
  • Cystadenocarcinoma, Serous / pathology
  • Disease-Free Survival
  • Female
  • Humans
  • Immunohistochemistry
  • MicroRNAs / biosynthesis*
  • MicroRNAs / genetics
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology
  • Reverse Transcriptase Polymerase Chain Reaction


  • MIRN29a microRNA, human
  • MicroRNAs