The first virally encoded cytochrome p450

J Virol. 2009 Aug;83(16):8266-9. doi: 10.1128/JVI.00289-09. Epub 2009 Jun 10.

Abstract

The genome sequence of the giant virus Acanthamoeba polyphaga mimivirus revealed the presence of two putative cytochrome P450 (CYP) genes. The product of one of the two predicted CYP genes (YP_143162) showed low-level homology to sterol 14-demethylase (CYP51) and contained a C-terminal polypeptide domain of unknown function. YP_143162 expression (without an N-terminal membrane binding domain) in Escherichia coli yields a CYP protein which gives a reduced CO difference maximum at 448 nm and was formally demonstrated as the first viral cytochrome P450. Analysis of binding of lipid and sterol substrates indicated no perturbation in CYP heme environment, and an absence of activity was seen when 14-methyl sterols were used as a substrate. The function of the CYP protein and its C-terminal domain remain unknown.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acanthamoeba / virology*
  • Amino Acid Sequence
  • Animals
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • DNA Viruses / chemistry
  • DNA Viruses / classification
  • DNA Viruses / enzymology*
  • DNA Viruses / genetics
  • Molecular Conformation
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Viral Proteins / chemistry
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Viral Proteins
  • Cytochrome P-450 Enzyme System