Abstract
Mutations in REPRESSOR OF SILENCING1 (ROS1) lead to the transcriptional gene silencing (TGS) of Pro(RD29A):LUC (LUCIFERASE) and Pro(35S):NPTII (Neomycin Phosphotransferase II) reporter genes. We performed a genetic screen to find suppressors of ros1 that identified two mutant alleles in the Arabidopsis (Arabidopsis thaliana) CHLOROPHYLL A/B BINDING PROTEIN UNDEREXPRESSED1 (CUE1) gene, which encodes a plastid inner envelope phosphoenolpyruvate/phosphate translocator. The cue1 mutations released the TGS of Pro(35S):NPTII and the transcriptionally silent endogenous locus TRANSCRIPTIONAL SILENCING INFORMATION in a manner that was independent of DNA methylation but dependent on chromatin modification. The cue1 mutations did not affect the TGS of Pro(RD29A):LUC in ros1, which was dependent on RNA-directed DNA methylation. It is possible that signals from chloroplasts help to regulate the epigenetic status of a subset of genomic loci in the nucleus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / drug effects
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Arabidopsis / genetics*
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Arabidopsis Proteins / metabolism*
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Biological Transport / drug effects
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Chromatin Immunoprecipitation
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DNA Methylation / drug effects
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Gene Expression Regulation, Plant / drug effects
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Gene Silencing* / drug effects
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Genes, Reporter
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Heterochromatin / metabolism
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Intracellular Membranes / drug effects
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Intracellular Membranes / metabolism*
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Lysine / metabolism
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Methyl Methanesulfonate / pharmacology
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Mutation / genetics
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Phosphates / metabolism
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Phosphoenolpyruvate / metabolism*
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Plastids / drug effects
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Plastids / metabolism*
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Promoter Regions, Genetic / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Seedlings / drug effects
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Seedlings / metabolism
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Transcription, Genetic* / drug effects
Substances
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Arabidopsis Proteins
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CUE1 protein, Arabidopsis
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Heterochromatin
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Phosphates
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RNA, Messenger
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Phosphoenolpyruvate
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Methyl Methanesulfonate
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Lysine