Exhaled breath condensate biomarkers in asbestos-related lung disorders

Respir Med. 2009 Aug;103(8):1091-7. doi: 10.1016/j.rmed.2009.04.009. Epub 2009 Jun 10.


Objectives: Asbestos induces generation of reactive oxygen and nitrogen species in laboratory studies. Several such species can be measured non-invasively in humans in exhaled breath condensate (EBC) but few have been evaluated. This study aimed to assess oxidative stress and lung inflammation in vivo.

Methods: Eighty six men were studied: sixty subjects with asbestos-related disorders (asbestosis: 18, diffuse pleural thickening (DPT): 16, pleural plaques (PPs): 26) and twenty six age- and gender-matched normal individuals.

Results: Subjects with asbestosis had raised EBC markers of oxidative stress compared with normal controls [8-isoprostane (geometric mean (95% CI) 0.51 (0.17-1.51) vs 0.07 (0.04-0.13) ng/ml, p<0.01); hydrogen peroxide (13.68 (8.63-21.68) vs 5.89 (3.99-8.69) microM, p<0.05), as well as increased EBC total protein (17.27 (10.57-28.23) vs 7.62 (5.13-11.34) microg/ml, p<0.05), and fractional exhaled nitric oxide (mean+/-SD) (9.67+/-3.26 vs 7.57+/-1.89ppb; p<0.05). EBC pH was lower in subjects with asbestosis compared with subjects with DPT (7.26+/-0.31 vs 7.53+/-0.24; p<0.05). There were no significant differences in exhaled carbon monoxide, EBC total nitrogen oxides and 3-nitrotyrosine between any of the asbestos-related disorders, or between these and controls.

Conclusion: In asbestos-related disorders, markers of inflammation and oxidative stress are significantly elevated in subjects with asbestosis compared with healthy individuals but not in pleural diseases.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Asbestos / toxicity*
  • Asbestosis / metabolism*
  • Asbestosis / physiopathology
  • Biomarkers / metabolism*
  • Dinoprost / analogs & derivatives
  • Dinoprost / metabolism
  • Forced Expiratory Volume / physiology
  • Humans
  • Lung Diseases / metabolism*
  • Lung Diseases / physiopathology
  • Male
  • Nitric Oxide / metabolism
  • Oxidative Stress / physiology*
  • Prognosis
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism


  • Biomarkers
  • Asbestos
  • 8-epi-prostaglandin F2alpha
  • Nitric Oxide
  • 3-nitrotyrosine
  • Tyrosine
  • Dinoprost