A genomic approach to yeast chronological aging

Methods Mol Biol. 2009;548:101-14. doi: 10.1007/978-1-59745-540-4_6.

Abstract

Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Culture Media / chemistry
  • Genomics / instrumentation
  • Genomics / methods*
  • Genomics / statistics & numerical data
  • Models, Genetic
  • Open Reading Frames
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development*
  • Sequence Deletion
  • Time Factors

Substances

  • Culture Media