Myeloperoxidase concentrations in EDTA-plasma of healthy subjects are discordant with concentrations in heparin-plasma and serum

Peter G Scheffer; Leonard P van der Zwan; Roger K Schindhelm; Hendrikus P A Vermue; Tom Teerlink

doi:10.1016/j.clinbiochem.2009.06.004

Myeloperoxidase concentrations in EDTA-plasma of healthy subjects are discordant with concentrations in heparin-plasma and serum

Clin Biochem. 2009 Sep;42(13-14):1490-2. doi: 10.1016/j.clinbiochem.2009.06.004. Epub 2009 Jun 12.

Authors

Peter G Scheffer¹, Leonard P van der Zwan, Roger K Schindhelm, Hendrikus P A Vermue, Tom Teerlink

Affiliation

¹ Metabolic Laboratory, Department of Clinical Chemistry, VU University Medical Center, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. p.scheffer@vumc.nl

PMID: 19524564
DOI: 10.1016/j.clinbiochem.2009.06.004

Abstract

Objectives: To examine the effect of blood anticoagulation type on the myeloperoxidase (MPO) concentration.

Design and methods: MPO was measured in EDTA-plasma and matched heparin-plasma and serum samples collected from healthy volunteers.

Results: MPO concentrations in heparin-plasma and serum were higher than in EDTA-plasma (both P<0.001). MPO in EDTA-plasma was not significantly correlated with MPO in either heparin-plasma or serum.

Conclusions: For MPO measurements, EDTA-plasma is the preferred specimen as it appears unaffected by ex vivo release of MPO from leukocytes.

MeSH terms

Adult
Anticoagulants / pharmacology*
Blood Preservation / methods
Edetic Acid / pharmacology
Female
Heparin / pharmacology
Humans
Male
Peroxidase / blood*
Plasma / drug effects*
Plasma / enzymology
Serum / drug effects*
Serum / enzymology
Temperature

Substances

Anticoagulants
Heparin
Edetic Acid
Peroxidase