Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Nat Methods. 2009 Jul;6(7):493-5. doi: 10.1038/nmeth.1342. Epub 2009 Jun 14.


We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agouti Signaling Protein / genetics*
  • Animals
  • Base Sequence
  • Cell Line
  • Crosses, Genetic
  • DNA Primers / genetics
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism*
  • Female
  • Gene Targeting
  • Genetic Techniques
  • Germ-Line Mutation
  • Hair Color / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL / classification
  • Mice, Inbred C57BL / genetics*
  • Mice, Knockout
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Polymorphism, Single Nucleotide
  • Pregnancy
  • Transplantation Chimera / genetics


  • Agouti Signaling Protein
  • DNA Primers
  • a protein, mouse