In vivo fluorescence imaging with high-resolution microlenses

Nat Methods. 2009 Jul;6(7):511-2. doi: 10.1038/nmeth.1339. Epub 2009 Jun 14.

Abstract

Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Dendritic Spines / metabolism
  • Dendritic Spines / ultrastructure
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Lenses*
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Neuromuscular Junction / metabolism
  • Neuromuscular Junction / ultrastructure
  • Pyramidal Cells / metabolism
  • Pyramidal Cells / ultrastructure
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism

Substances

  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins