A glycopeptide regulating stem cell fate in Arabidopsis thaliana

Nat Chem Biol. 2009 Aug;5(8):578-80. doi: 10.1038/nchembio.182. Epub 2009 Jun 7.

Abstract

The secreted peptide gene CLAVATA3 (CLV3) regulates stem cell fate in the shoot apical meristem in Arabidopsis thaliana plants, but the molecular structure of the active mature CLV3 peptide is controversial. Here, using nano-LC-MS/MS analysis of apoplastic peptides of A. thaliana plants overexpressing CLV3, we show that CLV3 is a 13-amino-acid arabinosylated glycopeptide. Post-translational arabinosylation of CLV3 is critical for its biological activity and high-affinity binding to its receptor CLV1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Chromatography, Liquid
  • Genes, Plant
  • Glycopeptides / chemistry
  • Glycopeptides / genetics
  • Glycopeptides / physiology*
  • Mass Spectrometry
  • Meristem / growth & development*
  • Meristem / metabolism
  • Molecular Sequence Data
  • Plant Shoots / growth & development
  • Plant Shoots / metabolism
  • Protein Binding
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases
  • Receptor Protein-Tyrosine Kinases / chemistry
  • Receptor Protein-Tyrosine Kinases / genetics
  • Receptor Protein-Tyrosine Kinases / physiology*

Substances

  • AT2G27250 protein, Arabidopsis
  • Arabidopsis Proteins
  • Glycopeptides
  • Receptor Protein-Tyrosine Kinases
  • CLV1 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases

Associated data

  • PubChem-Substance/58098229
  • PubChem-Substance/58098230
  • PubChem-Substance/58098231
  • PubChem-Substance/58098232