The accuracy, reliability and linearity in two hand-portable lactate analyzers, the Accutrend Lactate (AL) and the Lactate Pro (LP) versus the EBIO plus analyzer (EP) were evaluated. For accuracy, duplicate samples recorded on both the AL and LP revealed an overall average difference versus EP (P < 0.05). The limits of agreement between AL and EP were -0.7 to +1.0, and -1.3 to +1.5 mM between LP and EP. Reliability of AL and LP was assessed at different lactate concentrations; coefficient of variation ranged between 1.8 and 3.3% for AL and between 2.8 and 5.0% for LP. AL and LP had a good reliability for intra-, inter-analyzers, and between test strips (ICC r = 0.999). The linearity was determined versus the EP as reference. The slope coefficient of AL (1.0394) was closer to 1 than that of LP (1.1053). On these bases, AL and LP can be individually considered suitable for the sports research field.