Trichinella larvae isolated from the thigh muscle of a wild boar, Sus scrofa, captured from Gilan Province, northern Iran, was processed for DNA analysis. Polymerase chain reaction (PCR) for amplification of the 5S rDNA fragment demonstrated a 700 bp band on agarose gel. Analysis of DNA sequencing by BLAST confirmed the isolate as T. murrelli. This report constitutes the first recorded occurrence of T. murrelli in Asia, and also the first occurrence in a wild boar host.