Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells

Eur J Nutr. 2009 Aug;48(5):261-8. doi: 10.1007/s00394-009-0001-6. Epub 2009 Jun 17.


Background: Lycopene is a carotenoid whose biological activities and protective effect on prostate and breast cancer have been described, but little is known on its extra-intestinal metabolism and storage. While most alimentary lycopene is in all-trans configuration, in animal and human tissues approximately half of the lycopene is in cis isoforms.

Aim of study: Our object was to monitor the capacity of storage, isomerisation, and intracellular localization of all-trans and cis lycopene in hepatic stellate cells, which are the major sites of metabolism and storage of retinoids and carotenoids in the body.

Methods: We used the GRX cell line representative of murine hepatic stellate cells, incubated with 1-30 muM lycopene in culture medium. Analysis was done by high-performance liquid chromatography.

Results: Lycopene was able to induce expression of the lipocyte phenotype and it was internalized into GRX cells. Its cellular release only occurred in presence of albumin with a rapid initial decrease of intracellular lycopene. A corresponding increase in the culture medium was observed at 24 h. All-trans, 13-cis and 9-cis lycopene isoforms were identified in all the cell compartments. The membrane fraction contained the major part of lycopene, followed by the cytoplasmic fraction, lipid droplets and nuclei. The ratio between all-trans and cis isomers was approximately 2/1 in the majority parts of cell compartments.

Conclusions: This study identified a novel hepatic cell type able to store and isomerise lycopene. Liver can contribute to the serum and tissue equilibrium of cis/trans isomers of lycopene, and to participate in storage of lycopene under high extracellular concentration such as observed after the alimentary input.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Analysis of Variance
  • Animals
  • Anticarcinogenic Agents / analysis
  • Anticarcinogenic Agents / chemistry
  • Anticarcinogenic Agents / metabolism
  • Carotenoids / analysis
  • Carotenoids / chemistry*
  • Carotenoids / metabolism*
  • Cell Fractionation
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Hepatic Stellate Cells / cytology*
  • Hepatic Stellate Cells / metabolism*
  • Hepatocytes / cytology
  • Hepatocytes / metabolism
  • Isomerism
  • Lycopene
  • Mice
  • Phenotype


  • Anticarcinogenic Agents
  • Carotenoids
  • Lycopene