Abstract
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Helminth / blood
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Antigens, Helminth / analysis*
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Antigens, Helminth / chemistry
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Antigens, Helminth / immunology
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Brugia pahangi / chemistry*
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Brugia pahangi / immunology
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Electrophoresis, Gel, Two-Dimensional
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Electrophoresis, Polyacrylamide Gel
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Elephantiasis, Filarial / blood
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Elephantiasis, Filarial / immunology
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Elephantiasis, Filarial / parasitology
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Gerbillinae
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Helminth Proteins / analysis*
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Helminth Proteins / chemistry
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Helminth Proteins / immunology
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Humans
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Image Processing, Computer-Assisted
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Immunoblotting
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Molecular Sequence Data
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Proteomics
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antibodies, Helminth
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Antigens, Helminth
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Helminth Proteins