Three-dimensional structure of the two-peptide bacteriocin plantaricin JK

Peptides. 2009 Sep;30(9):1613-21. doi: 10.1016/j.peptides.2009.06.010. Epub 2009 Jun 16.

Abstract

The three-dimensional structures of the two peptides, PlnJ and PlnK, that constitutes the two-peptide bacteriocin plantaricin JK have been solved in water/TFE and water/DPC-micellar solutions using nuclear magnetic resonance (NMR) spectroscopy. PlnJ, a 25 residue peptide, has an N-terminal amphiphilic alpha-helix between Trp-3 and Tyr-15. The 32 residues long PlnK forms a central amphiphilic alpha-helix between Gly-9 and Leu-24. Measurements of the effect on anti-microbial activity of single glycine replacements in PlnJ and PlnK show that Gly-13 and Gly-17 in both peptides are very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. In variants where other glycine residues, Gly-20 in PlnJ and Gly-7, Gly-9, Gly-24 and Gly-25 in PlnK, were replaced, the activity was reduced less than 10-fold. It is proposed that the detrimental effect on activity when exchanging Gly-13 and Gly-17 in PlnJ and PlnK is a result of reduced ability of the two peptides to interact through the GxxxG-motifs constituting Gly-13 and Gly-17.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Amino Acid Substitution / genetics
  • Bacteriocins / biosynthesis
  • Bacteriocins / chemistry*
  • Bacteriocins / pharmacokinetics
  • Bacteriocins / pharmacology
  • Cell Proliferation / drug effects
  • Circular Dichroism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Glycine / genetics
  • Lactobacillus / drug effects
  • Lactobacillus plantarum / drug effects
  • Lactobacillus plantarum / genetics
  • Liposomes / chemistry
  • Micelles
  • Models, Molecular
  • Nitrogen Isotopes / metabolism
  • Nuclear Magnetic Resonance, Biomolecular
  • Phosphorylcholine / analogs & derivatives
  • Phosphorylcholine / chemistry
  • Protein Structure, Tertiary
  • Protein Subunits / biosynthesis
  • Protein Subunits / chemistry
  • Protein Subunits / pharmacology
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / pharmacology
  • Trifluoroethanol / chemistry

Substances

  • Bacteriocins
  • Liposomes
  • Micelles
  • Nitrogen Isotopes
  • Protein Subunits
  • Recombinant Proteins
  • plantaricin JK
  • Phosphorylcholine
  • dodecylphosphocholine
  • Trifluoroethanol
  • Glycine