IL-12 and TNF-alpha production by dendritic cells stimulated with Schistosoma mansoni schistosomula tegument is TLR4- and MyD88-dependent

Immunol Lett. 2009 Jun 30;125(1):72-7. doi: 10.1016/j.imlet.2009.06.004. Epub 2009 Jun 17.

Abstract

Schistosoma mansoni schistosomula are the most susceptible parasite life stage to host immune system attack. Complex host-parasite interactions take place on Schistosoma tegument, which is a unique double membrane structure involved in nutrition and immune evasion. Herein, we have demonstrated that schistosomula tegument (Smteg) activates Dendritic cells to produce IL-12p40, TNF-alpha and also to up-regulate the co-stimulatory molecules CD40 and CD86. Moreover, using DCs derived from MyD88-, TLR2-, TLR4- and TLR9-deficient mice we have shown that the ability of Smteg to activate DCs to produce IL-12 and TNF-alpha involves TLR4/Smteg interaction and MyD88 signaling pathway. Finally, our findings lead us to conclude that TLR4 is a key receptor involved in Smteg induction of pro-inflammatory cytokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B7-2 Antigen / immunology
  • B7-2 Antigen / metabolism
  • CD40 Antigens / immunology
  • CD40 Antigens / metabolism
  • Dendritic Cells / immunology*
  • Host-Parasite Interactions / immunology*
  • Interleukin-12 Subunit p40 / biosynthesis*
  • Interleukin-12 Subunit p40 / immunology
  • Lipopolysaccharides / pharmacology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myeloid Differentiation Factor 88 / genetics
  • Myeloid Differentiation Factor 88 / immunology*
  • Schistosoma mansoni / immunology*
  • Signal Transduction / immunology
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / immunology*
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / immunology
  • Up-Regulation / immunology

Substances

  • B7-2 Antigen
  • CD40 Antigens
  • Interleukin-12 Subunit p40
  • Lipopolysaccharides
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • Toll-Like Receptor 4
  • Tumor Necrosis Factor-alpha