c-Maf is essential for the F4/80 expression in macrophages in vivo

Gene. 2009 Sep 15;445(1-2):66-72. doi: 10.1016/j.gene.2009.06.003. Epub 2009 Jun 17.


c-Maf, which is one of the large Maf transcription factors, can bind to Maf recognition element (MARE) and activates transcription of target genes. Although c-Maf is expressed in macrophages and directly regulates the expression of interleukin-10, detailed information regarding its function in the null mutant phenotype of tissue macrophages remain unknown. In this study, we demonstrated that c-Maf is specifically expressed in the F4/80 positive fetal liver and adult macrophages. The expression of F4/80, which is a tissue macrophage-specific seven trans-membrane receptor, was dramatically suppressed in the c-Maf-deficient macrophage, whereas the expression of Mac-1 was not affected, suggesting that c-Maf is not necessary for the lineage commitment of macrophages. Luciferase reporter and EMSA showed that c-Maf directly regulates the expression of F4/80 by interacting with the half-MARE site of the F4/80 promoter. These results suggest that c-Maf is required for the F4/80 expression in macrophages in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / genetics*
  • Antigens, Differentiation / metabolism
  • Base Sequence
  • Cells, Cultured
  • Embryo, Mammalian
  • Gene Expression Regulation
  • Liver / embryology
  • Liver / metabolism
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Proto-Oncogene Proteins c-maf / genetics
  • Proto-Oncogene Proteins c-maf / metabolism
  • Proto-Oncogene Proteins c-maf / physiology*
  • Sequence Homology, Nucleic Acid


  • Antigens, Differentiation
  • Maf protein, mouse
  • Proto-Oncogene Proteins c-maf
  • monocyte-macrophage differentiation antigen