Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees

Mycol Res. 2009 Sep;113(Pt 9):973-80. doi: 10.1016/j.mycres.2009.06.001. Epub 2009 Jun 17.


Three endophytic yeast, one isolated from stems of wild cottonwood (Populus trichocarpa), two from stems of hybrid poplar (P. trichocarpaxPopulus deltoides), were characterized by analyzing three ribosomal genes, the small subunit (18S), internal transcribed spacer (ITS), and D1/D2 region of the large subunit (26S). Phenotypic characteristics of the yeast isolates were also obtained using a commercial yeast identification kit and used for assisting the species identification. The isolate from wild cottonwood was identified to be closest to species Rhodotorula graminis. The two isolates from hybrid poplar were identified to be species Rhodotorula mucilaginosa. In addition, the three yeast isolates were observed to be able to produce indole-3-acetic acid (IAA), a phytohormone which can promote plant growth, when incubated with l-tryptophan. To our knowledge, the yeast strains presented in this study were the first endophytic yeast strains isolated from species of Populus.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carbon / metabolism
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • DNA, Intergenic / genetics
  • Genes, rRNA
  • Indoleacetic Acids / metabolism*
  • Plant Stems / microbiology
  • Populus / microbiology*
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal, 18S / genetics
  • Rhodotorula / cytology
  • Rhodotorula / genetics
  • Rhodotorula / isolation & purification
  • Rhodotorula / metabolism*
  • Sequence Analysis, DNA
  • Species Specificity


  • DNA, Fungal
  • DNA, Intergenic
  • Indoleacetic Acids
  • RNA, Ribosomal
  • RNA, Ribosomal, 18S
  • RNA, ribosomal, 26S
  • indoleacetic acid
  • Carbon