Chemiluminescent substrates increase sensitivity of antigen detection in western blots

Biotechniques. 1991 Jul;11(1):14-6.


Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.

MeSH terms

  • Antigens, Bacterial / analysis*
  • Blotting, Western / methods*
  • Colorimetry
  • Escherichia coli / immunology*
  • Indoles
  • Luminescent Measurements
  • Nitroblue Tetrazolium


  • Antigens, Bacterial
  • Indoles
  • Nitroblue Tetrazolium
  • 5-bromo-4-chloro-3-indoxyl phosphate