Identification of a stable chemerin analog with potent activity toward ChemR23

Peptides. 2009 Aug;30(8):1529-38. doi: 10.1016/j.peptides.2009.05.030. Epub 2009 Jun 18.


Chemerin is a novel peptide that was identified as a natural ligand for ChemR23. As it has been reported to be involved in the regulation of immune responses and adipogenesis, chemerin may have a variety of physiological functions. Chemerin is synthesized as a precursor (prochemerin) and is proteolytically activated and inactivated in sequential steps, which control its physiological roles in a coordinated manner. Chemerin-9 (chemerin148-156) was previously identified as the smallest peptide with low nanomolar potency. However, like mature chemerin, chemerin-9 is rapidly degraded and inactivated in plasma, which has limited the use of chemerin-9 in in vivo experiments. In order to identify stable chemerin analogs that facilitate in vivo studies, we synthesized a series of chemerin-9 analogs and examined intrinsic activity and metabolic stability. We identified an agonistic and metabolically stable chemerin-9 analog (d-Tyr(147)-[d-Ser(151), d-Ala(154), Tic(155)]chemerin148-156) that shows enhanced plasma exposure with prolonged half-life in mice upon intraperitoneal administration. Improvement of metabolic stability resulted in a reduction in the plasma free fatty acid levels in fasted mice, which cannot be accomplished by unstable-mouse chemerin-9. This reduction in plasma free fatty acids reflects the anti-lipolysis activity of chemerin-9 and analogs in mouse primary adipocytes. The discovery of a metabolically stable chemerin analog will facilitate investigation of the pharmacological roles of chemerin in vivo. Moreover, this stable chemerin analog might provide new therapeutic approaches to inflammatory diseases such as asthma and metabolic disorders such as obesity and diabetes where ChemR23 activation may be of benefit.

MeSH terms

  • 3T3 Cells
  • Adipocytes / drug effects
  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Chemokines
  • Chemotactic Factors / blood*
  • Chemotactic Factors / chemistry
  • Chemotactic Factors / metabolism
  • Chemotactic Factors / pharmacology
  • Cricetinae
  • Cricetulus
  • Intercellular Signaling Peptides and Proteins / blood
  • Intercellular Signaling Peptides and Proteins / chemistry
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Intercellular Signaling Peptides and Proteins / pharmacology
  • Mass Spectrometry
  • Mice
  • Molecular Sequence Data
  • Peptides / blood
  • Peptides / chemistry
  • Peptides / metabolism*
  • Peptides / pharmacology
  • Receptors, Chemokine / metabolism*


  • Chemokines
  • Chemotactic Factors
  • Intercellular Signaling Peptides and Proteins
  • Peptides
  • Receptors, Chemokine
  • chemerin protein, mouse