Mass spectrometric quantification of MIF-1 in mouse brain by multiple reaction monitoring

Peptides. 2009 Jul;30(7):1276-81. doi: 10.1016/j.peptides.2009.04.004. Epub 2009 Apr 11.

Abstract

MIF-1 (Pro-Leu-Gly-NH(2)) has potent therapeutic effects in depression and Parkinson's disease, but its CNS sites of production are not yet clear. In this study, the concentration of MIF-1 in different brain regions was measured by the multiple reaction monitoring technique on a 4000 QTRAP mass spectrometer. The limit of quantification was 300 fg of MIF-1, and limit of detection was 60 fg. The low molecular weight fractions of tissue homogenates from different regions of mouse brain were analyzed. The concentration of MIF-1 ranged from 22+/-3 fg/microg protein in cerebral cortex to 930+/-60 fg/microg protein in the hypothalamus. Moderate concentrations were also detected in all other regions tested, including the striatum, thalamus, and hippocampus. By incubation of stable isotope-labeled oxytocin with tissue preparations, it was also confirmed that oxytocin at least partially contributed to the production of MIF-1 in the hypothalamus by action of peptidases. Regional differences were also found. The results are the first to show the ultrasensitive quantification of MIF-1 in different brain regions, and support the neuromodulatory actions of MIF-1 in the striatum.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Chromatography, High Pressure Liquid / methods
  • Female
  • Hypothalamus / metabolism
  • MSH Release-Inhibiting Hormone / metabolism*
  • Mice
  • Oxytocin / metabolism
  • Tandem Mass Spectrometry

Substances

  • Oxytocin
  • MSH Release-Inhibiting Hormone