Mouse adenovirus type 1 infection of macrophages

Virology. 2009 Aug 1;390(2):307-14. doi: 10.1016/j.virol.2009.05.025. Epub 2009 Jun 21.

Abstract

Mouse adenovirus type 1 (MAV-1) causes acute and persistent infections in mice, with high levels of virus found in the brain, spinal cord and spleen in acute infections. MAV-1 infects endothelial cells throughout the mouse, and monocytes/macrophages have also been implicated as targets of the virus. Here we determined the extent and functional importance of macrophage infection by MAV-1. Bone marrow-derived macrophages expressed MAV-1 mRNAs and proteins upon ex vivo infection. Adherent peritoneal macrophages from infected mice expressed viral mRNAs and produced infectious virus. Infected chemokine (C-C motif) receptor 2 (CCR2) knockout mice, which are defective for macrophage recruitment, did not show differences in survival or MAV-1 load compared to controls. In contrast, macrophage depletion using clodronate-loaded liposomes resulted in increased virus replication in spleens of a MAV-1-resistant mouse strain, BALB/cJ. Thus macrophages serve both as targets of infection and as effectors of the host response.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae / immunology
  • Adenoviridae / pathogenicity*
  • Adenoviridae Infections / immunology
  • Adenoviridae Infections / veterinary*
  • Adenoviridae Infections / virology
  • Animals
  • Macrophages / immunology
  • Macrophages / virology*
  • Macrophages, Peritoneal / microbiology
  • Macrophages, Peritoneal / virology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • RNA, Messenger / biosynthesis
  • RNA, Viral / biosynthesis
  • Receptors, CCR2 / deficiency
  • Spleen / virology
  • Survival Analysis
  • Viral Proteins / biosynthesis

Substances

  • Ccr2 protein, mouse
  • RNA, Messenger
  • RNA, Viral
  • Receptors, CCR2
  • Viral Proteins