Therapeutic effects of topical application of ozone on acute cutaneous wound healing

J Korean Med Sci. 2009 Jun;24(3):368-74. doi: 10.3346/jkms.2009.24.3.368. Epub 2009 Jun 12.


This study was undertaken to evaluate the therapeutic effects of topical ozonated olive oil on acute cutaneous wound healing in a guinea pig model and also to elucidate its therapeutic mechanism. After creating full-thickness skin wounds on the backs of guinea pigs by using a 6 mm punch biopsy, we examined the wound healing effect of topically applied ozonated olive oil (ozone group), as compared to the pure olive oil (oil group) and non-treatment (control group). The ozone group of guinea pig had a significantly smaller wound size and a residual wound area than the oil group, on days 5 (P<0.05) and 7 (P<0.01 and P<0.05) after wound surgery, respectively. Both hematoxylin-eosin staining and Masson-trichrome staining revealed an increased intensity of collagen fibers and a greater number of fibroblasts in the ozone group than that in the oil group on day 7. Immunohistochemical staining demonstrated upregulation of platelet derived growth factor (PDGF), transforming growth factor-beta (TGF-beta) and vascular endothelial growth factor (VEGF) expressions, but not fibroblast growth factor expression in the ozone group on day 7, as compared with the oil group. In conclusion, these results demonstrate that topical application of ozonated olive oil can accelerate acute cutaneous wound repair in a guinea pig in association with the increased expression of PDGF, TGF-beta, and VEGF.

Keywords: Collagen; Ozone; Wound Healing.

Publication types

  • Comparative Study

MeSH terms

  • Acute Disease
  • Administration, Topical
  • Animals
  • Female
  • Guinea Pigs
  • Olive Oil
  • Ozone / administration & dosage
  • Ozone / therapeutic use*
  • Plant Oils / administration & dosage
  • Plant Oils / therapeutic use
  • Platelet-Derived Growth Factor / metabolism
  • Skin / drug effects*
  • Skin / pathology
  • Transforming Growth Factor beta / metabolism
  • Vascular Endothelial Growth Factor A / metabolism
  • Wound Healing / drug effects*


  • Olive Oil
  • Plant Oils
  • Platelet-Derived Growth Factor
  • Transforming Growth Factor beta
  • Vascular Endothelial Growth Factor A
  • Ozone