Steric constraints dependent on nucleobase pair orientation vary in different DNA polymerase active sites

Chembiochem. 2009 Jul 6;10(10):1630-3. doi: 10.1002/cbic.200900123.


Finding the right fit: Herein, we report on the development of novel steric probes and present initial insights into their interplay with DNA polymerases. Our findings provide experimental evidence for varied enzyme-substrate interactions that might account for the varied selectivity previously observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing
  • Catalytic Domain*
  • DNA Polymerase I / chemistry*
  • DNA Polymerase I / metabolism
  • DNA Polymerase beta / chemistry*
  • DNA Polymerase beta / metabolism
  • Kinetics
  • Nucleotides / chemistry*
  • Substrate Specificity


  • Nucleotides
  • DNA Polymerase I
  • DNA Polymerase beta