Activation and signaling status of human lamina propria T lymphocytes

Gastroenterology. 1991 Dec;101(6):1529-36. doi: 10.1016/0016-5085(91)90388-2.


In this study, proliferative responses of human lamina propria T lymphocytes were examined in vitro. The response of lamina propria T lymphocytes to Sepharose-bound anti-CD3 antibody plus interleukin 2 was significantly lower than the response of autologous peripheral blood T lymphocytes, whereas the responses of lamina propria T lymphocytes to anti-T11(2/3) antibodies plus sheep erythrocytes or anti-CD28 antibody plus interleukin 2 were largely preserved. After coculture with mucosa supernatant, peripheral blood T lymphocytes showed a similar pattern of reactivity as lamina propria T lymphocytes. This reduced reactivity to T-cell antigen receptor stimulation appears to exist at the level of signal transduction, because triggering of CD3 induces low amounts of intracellular inositol 1,4,5-triphosphate and no free calcium increase in lamina propria T lymphocytes when compared with peripheral blood T lymphocytes. This study indicates that the antigen receptor-dependent activation pathway of lamina propria T lymphocytes for proliferation is down-regulated by intestinal mucosa derived factor(s) and that the alternative pathways mediated by CD2 or CD28 are largely preserved. Based on previous data that lamina propria T lymphocytes can provide help to B cells, it is possible that these alternative activation pathways play an important role in T-B cell interaction in the gut.

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / immunology
  • Antigens, CD / physiology
  • Blood / immunology
  • Cell Division / physiology
  • Colon / immunology
  • Humans
  • In Vitro Techniques
  • Interleukin-2
  • Intestinal Mucosa / immunology*
  • Lymphocyte Activation / immunology*
  • Signal Transduction* / immunology*
  • T-Lymphocytes / physiology*


  • Antibodies, Monoclonal
  • Antigens, CD
  • Interleukin-2