Transcriptional coactivator EDF-1 is required for PPARgamma-stimulated adipogenesis

Cell Mol Life Sci. 2009 Aug;66(16):2733-42. doi: 10.1007/s00018-009-0069-4. Epub 2009 Jun 25.


Peroxisome proliferator-activated receptor-gamma (PPARgamma) is essential for adipogenesis. Since EDF-1 is a cofactor of PPARgamma, we investigated the molecular cross-talk between EDF-1 and PPARgamma in adipogenesis. While EDF-1 was not modulated during differentiation of 3T3-L1 cells, it co-immunoprecipitated with PPARgamma. Silencing EDF-1 by shRNAs inhibited the differentiation in adipocytes of 3T3-L1 cells, as detected by the staining of intracellular triglycerides and the expression of the PPARgamma target gene aP2. Accordingly, we found that anti-EDF-1 shRNAs decreased ligand dependent activation of PPARgamma in 3T3-L1 transiently transfected with a vector expressing luciferase under the control of a PPARgamma responsive consensus. To rule out that this inhibition is due to the concomitant downregulation of PPARgamma levels, we overexpressed PPARgamma in 3T3-L1 silencing EDF-1 and found a decrease of ligand dependent activation of PPARgamma, in spite of the high amounts of PPARgamma. These results demonstrate that EDF-1 is required for PPARgamma transcriptional activation during 3T3-L1 differentiation.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Adipogenesis / genetics*
  • Animals
  • Calmodulin-Binding Proteins / genetics
  • Calmodulin-Binding Proteins / metabolism
  • Calmodulin-Binding Proteins / physiology*
  • Cell Differentiation / genetics
  • Ligands
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • PPAR gamma / physiology*
  • Protein Transport
  • RNA Interference
  • Transcriptional Activation


  • Calmodulin-Binding Proteins
  • Edf1 protein, mouse
  • Ligands
  • PPAR gamma