A system of calibrating microtomography for use in caries research

Caries Res. 2009;43(4):314-21. doi: 10.1159/000226230. Epub 2009 Jun 24.

Abstract

Desktop microcomputed tomography (micro-CT) offers a non-invasive 3-dimensional analysis of structures and their physical properties. To date, the use of micro-CT has mostly involved qualitative observations, with the extent of quantitative analysis relying on automated internal calibration by the micro-CT control software. However, the value of such calibration is limited by machine drift. For an accurate quantitative use of micro-CT, it is recognized that external means of calibration are needed. A novel system of calibration standards, also known as 'phantoms', is presented. A range of low mineral concentration phantoms involving triethylene glycol dimethacrylate/glycerolate dimethacrylate resin mixed with commercial pure hydroxyapatite (HAP), from 0.07 to 1.05 g/cm(3), was fabricated. Sintered HAP was impregnated with the same resin, producing phantoms with medium-level mineral concentrations up to 1.90 g/cm(3). These phantoms were easy to create, proved accurate and stable with repeated use, and were found to mimic the composite nature of dental enamel and dentine structures under investigation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Bone Density
  • Calibration
  • Cattle
  • Dental Caries / diagnostic imaging*
  • Dental Enamel / diagnostic imaging*
  • Humans
  • Hydroxyapatites / analysis*
  • Phantoms, Imaging / standards*
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • X-Ray Microtomography / instrumentation*
  • X-Ray Microtomography / methods

Substances

  • Hydroxyapatites