Pattern formation in the Drosophila eye disc

Int J Dev Biol. 2009;53(5-6):795-804. doi: 10.1387/ijdb.072483jr.


Differentiation of the Drosophila compound eye from the eye imaginal disc is a progressive process: columns of cells successively differentiate in a posterior to anterior sequence, clusters of cells form at regularly spaced intervals within each column, and individual photoreceptors differentiate in a defined order within each cluster. The progression of differentiation across the eye disc is driven by a positive autoregulatory loop of expression of the secreted molecule Hedgehog, which is temporally delayed by the intercalation of a second signal, Spitz. Hedgehog refines the spatial position at which each column initiates its differentiation by inducing secondary signals that act over different ranges to control the expression of positive and negative regulators. The position of clusters within each column is controlled by secreted inhibitory signals from clusters in the preceding column, and a single founder neuron, R8, is singled out within each cluster by Notch-mediated lateral inhibition. R8 then sequentially recruits surrounding cells to differentiate by producing a short-range signal, Spitz, which induces a secondary short-range signal, Delta. Intrinsic transcription factors act in combination with these two signals to produce cell-type diversity within the ommatidium. The Hedgehog and Spitz signals are transported along the photoreceptor axons and reused within the brain as long-range and local cues to trigger the differentiation and assembly of target neurons.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Body Patterning*
  • Cell Differentiation
  • Developmental Biology / methods*
  • Drosophila / embryology*
  • Drosophila / physiology
  • Drosophila Proteins / metabolism
  • Epidermal Growth Factor / metabolism
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / metabolism
  • Models, Biological
  • Neurons / physiology
  • Photoreceptor Cells, Invertebrate / cytology*
  • Photoreceptor Cells, Invertebrate / pathology
  • Transcription Factors / metabolism


  • Drosophila Proteins
  • Hedgehog Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Transcription Factors
  • delta protein
  • spi protein, Drosophila
  • Epidermal Growth Factor