Effects of N-acetylcysteine on regeneration following partial hepatectomy in rats with nonalcoholic fatty liver disease

Surg Today. 2009;39(7):592-7. doi: 10.1007/s00595-008-3930-4. Epub 2009 Jun 28.

Abstract

Purpose: To investigate the effect of N-acetylcysteine on regeneration following partial hepatectomy in rats with nonalcoholic fatty liver disease (NAFLD).

Methods: N-Acetylcysteine was given to seven rats with NAFLD (group 1); physiological saline was given to seven rats with NAFLD (group 2); and physiological saline was given to seven rats with a normal liver (group 3). We performed two-thirds hepatectomy in all rats and removed the remnant liver tissue 48 h later to measure the mitotic index (MI), proliferating cell nuclear antigen, glutathione (GSH), and malondialdehyde (MDA) levels.

Results: Mitotic index values were significantly higher in group 1 than in groups 2 and 3, and higher in group 3 than in group 2. Proliferating cell nuclear antigen values were significantly higher in group 1 than in group 2, but no significant difference was found in comparison with group 3. Glutathione values in group 1 were significantly higher than in group 2 and MDA values in group 1 were lower than in group 2. There was no significant difference between groups 1 and 3 in GSH and MDA values, in both the two-thirds hepatectomy and 48-h tissues.

Conclusions: N-Acetylcysteine enhanced regeneration after partial hepatectomy in rats with NAFLD. We believe that it exerted this effect through its influence on oxidative stress.

MeSH terms

  • Acetylcysteine / pharmacology*
  • Animals
  • Antioxidants / pharmacology*
  • Fatty Liver / surgery*
  • Hepatectomy*
  • Liver Regeneration / drug effects*
  • Male
  • Mitotic Index
  • Oxidative Stress / drug effects
  • Rats
  • Rats, Wistar

Substances

  • Antioxidants
  • Acetylcysteine