Protein composition of oil bodies from mature Brassica napus seeds

Proteomics. 2009 Jun;9(12):3268-84. doi: 10.1002/pmic.200800449.


Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Brassica napus / chemistry*
  • Brassica napus / genetics
  • Brassica rapa / chemistry
  • Brassica rapa / genetics
  • Calcium-Binding Proteins / analysis
  • Immunoblotting
  • Inclusion Bodies / chemistry*
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / analysis
  • Protein Processing, Post-Translational
  • Seed Storage Proteins / analysis*
  • Seed Storage Proteins / chemistry
  • Seed Storage Proteins / genetics
  • Seeds / chemistry*
  • Sequence Alignment


  • Calcium-Binding Proteins
  • Plant Proteins
  • Seed Storage Proteins
  • caleosin protein, Sesamum indicum
  • oleosin protein, Brassica napus