The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors

Dev Biol. 2009 Sep 1;333(1):67-77. doi: 10.1016/j.ydbio.2009.06.024. Epub 2009 Jun 27.


Most tissues display several features of cellular polarization. Besides the ubiquitous epithelial polarization in the Apical-Basal (A/B) axis, many epithelia (and associated organs) display a Planar Cell Polarization (PCP). Recently, a crosstalk between the PCP and A/B polarity determinants has been suggested, i.e. the activity or stability of the PCP factor Frizzled is regulated by the A/B determinants aPKC and Bazooka in the Drosophila eye. We have systematically investigated genetic and physical interactions between the Drosophila A/B factors and the core PCP component Strabismus (Stbm)/Van Gogh (Vang). The A/B determinant Scribble was found to interact both genetically and physically with Stbm/Vang. We demonstrate that Scribble binds Stbm/Vang through its PDZ domain 3 and that it cooperates with Stbm/Vang in PCP establishment. Our data indicate that Scribble, in addition to its role in A/B polarity, has a distinct requirement in PCP establishment in the Drosophila eye and wing. We define a scribble allele that is largely PCP specific. Our data show that Scribble is part of the Stbm/Vang PCP complex and further suggest that it might act as an effector of Stbm/Vang during PCP establishment.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Body Patterning / physiology
  • Cell Polarity / physiology
  • Compound Eye, Arthropod / cytology
  • Compound Eye, Arthropod / growth & development*
  • Compound Eye, Arthropod / metabolism
  • Drosophila / cytology
  • Drosophila / growth & development*
  • Drosophila / metabolism
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mutation
  • Protein Binding
  • Wings, Animal / cytology
  • Wings, Animal / growth & development*
  • Wings, Animal / metabolism


  • Drosophila Proteins
  • Membrane Proteins
  • Scrib protein, Drosophila
  • Vang protein, Drosophila