Developing compact multiphoton systems using femtosecond fiber lasers

J Biomed Opt. 2009 May-Jun;14(3):030508. doi: 10.1117/1.3153842.

Abstract

We implement a fiber-delivered compact femtosecond fiber laser at 1,030-nm wavelength in multiphoton imaging. The laser pulse duration is 150 fs, the average power is 200 mW, and the repetition rate is 40 MHz. The laser measures 200 x 160 x 45 mm in size and its output is delivered through a photonic bandgap fiber. Intrinsic second-harmonic generation signal is excited from rat tail tendon and human skin samples. Two-photon excited fluorescence signal is obtained from human skin tissues stained with exogenous fluorophore. Our results show that femtosecond fiber lasers at 1030-nm wavelength have significant potential in developing compact, all-fiber-based, portable multiphoton systems and endoscopes.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acoustics
  • Animals
  • Fiber Optic Technology / instrumentation
  • Fiber Optic Technology / methods*
  • Humans
  • Lasers
  • Microscopy, Fluorescence, Multiphoton / instrumentation
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Rats
  • Skin / anatomy & histology
  • Tail / anatomy & histology
  • Tendons / anatomy & histology