Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

J Biomed Opt. 2009 May-Jun;14(3):034049. doi: 10.1117/1.3147385.


Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics
  • Cytokinesis / physiology
  • Holography / methods*
  • Linear Models
  • Microscopy / methods*
  • Mutation
  • Schizosaccharomyces / cytology*
  • Schizosaccharomyces / growth & development
  • Schizosaccharomyces pombe Proteins / genetics


  • Cell Cycle Proteins
  • Schizosaccharomyces pombe Proteins
  • cdc16 protein, S pombe