Performance of the Epstein-Barr virus and herpes simplex virus immunoglobulin m assays on the liaison platform with sera from patients displaying acute parvovirus B19 infection

Clin Vaccine Immunol. 2009 Aug;16(8):1247-8. doi: 10.1128/CVI.00142-09. Epub 2009 Jul 1.


Acute parvovirus B19 infection has been reported to cause false-positive results frequently in the Epstein-Barr (EBV) and herpes simplex virus (HSV) immunoglobulin M (IgM) assays from DiaSorin performed on the Liaison platform. We tested 65 sera from patients with a presumptive or conclusive diagnosis of acute parvovirus B19 infection in both assays and obtained no false-positive results in the EBV IgM test and 10.4% nonspecific reactivities in the HSV IgM assay. Our data support the specificity of both assays in this clinical setting.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Epstein-Barr Virus Infections / diagnosis*
  • False Positive Reactions
  • Female
  • Herpes Simplex / diagnosis*
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Immunoglobulin M / blood*
  • Male
  • Middle Aged
  • Parvoviridae Infections / complications*
  • Parvoviridae Infections / virology
  • Parvovirus B19, Human / isolation & purification*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Simplexvirus / immunology*
  • Young Adult


  • Immunoglobulin M
  • Reagent Kits, Diagnostic