Determination of kappa and lambda light chains in serum immunoglobulins G, A and M

Ann Clin Biochem. 1991 Sep;28 ( Pt 5):461-6. doi: 10.1177/000456329102800507.


An enzyme-linked immunosorbent assay is described for measuring kappa and lambda light chains within each of the serum immunoglobulin classes G, A and M. The detection limit was 0.06 U/L for total IgG, IgG kappa and IgG lambda, 0.2 U/L for total IgA, IgA kappa and IgA lambda and 0.5 U/L for total IgM, IgM kappa and IgM lambda. The concentrations of kappa plus lambda light chains from the three different immunoglobulins correlated well within those of total immunoglobulin G, A and M as measured by enzyme-linked immunosorbent assay or by immunonephelometry. Adult values for the kappa/lambda light chain ratio were found to be 2.0 for IgG kappa/lambda, 1.1 for IgA kappa/lambda and 1.7 for IgM kappa/lambda.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Humans
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Immunoglobulin kappa-Chains / blood*
  • Immunoglobulin lambda-Chains / blood*
  • Infant
  • Infant, Newborn
  • Male
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulin kappa-Chains
  • Immunoglobulin lambda-Chains