Constitutive activation of NF-kB in human thymocytes

Biochem Biophys Res Commun. 1991 Nov 27;181(1):80-6. doi: 10.1016/s0006-291x(05)81384-1.


NF-kB is a eukaryotic transcription regulatory factor. In T cells and T cell lines, NF-kB is bound to a cytoplasmic proteic inhibitor, the IkB. Treatment of T cells with mitogens (phorbol esters) or cytokines (TNF alpha) induces NF-kB nuclear translocation and the subsequent expression of NF-kB dependent T cell genes. Here we examined the activation of NF-kB in human T cell thymic progenitors. We report differences in (Ca2+)i requirement for NF-kB activation in thymocytes as compared to mature T cells. Furthermore, our results indicated that thymocytes have a constitutively active form of NF-kB, suggesting that they are activated in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding, Competitive
  • Cell Line
  • Humans
  • Lymphocyte Activation
  • Molecular Sequence Data
  • NF-kappa B / metabolism*
  • Nucleoproteins / metabolism
  • Oligodeoxyribonucleotides
  • Recombinant Proteins / metabolism
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thymus Gland / immunology


  • NF-kappa B
  • Nucleoproteins
  • Oligodeoxyribonucleotides
  • Recombinant Proteins
  • Tetradecanoylphorbol Acetate