Protecting effect of phytoncide solution, on normal human dermal fibroblasts against reactive oxygen species

J Oleo Sci. 2009;58(8):429-36. doi: 10.5650/jos.58.429.

Abstract

Four types of phytoncide solutions (A-Type, AB-Type, D-Type and G-Type) was evaluated for reduction of cell damage induced by oxidative stress, ultraviolet A (UVA), ultraviolet B (UVB), hydroxyperoxide (H2O2) and t-butyl-hydroperoxide (t-BHP); stimulation of collagen synthesis against UVA irradiation; and inhibition of matrix metalloproteinase-1 (MMP-1) activity induced by UVA in human normal dermal fibroblasts and human reconstituted skin model. The A-Type, AB-Type, D-Type and G-Type of phytoncide solutions pretreatment resulted in significant protection against cell damage induced by UVB, UVA, H2O2 and t-BHP. The amount of type I collagen following UVA irradiation was increased by treatment with phytoncide solutions in a concentration-dependent manner. On the other hand, phytoncide solutions also suppressed the excess MMP-1 irradiated UVA in a concentration-dependent manner. These effects of G-type solution were superior to those of other types solutions.

MeSH terms

  • Dose-Response Relationship, Drug
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism*
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Matrix Metalloproteinase 1 / metabolism
  • Matrix Metalloproteinase Inhibitors
  • Oxidative Stress / drug effects*
  • Plant Extracts / pharmacology*
  • Poaceae / chemistry
  • Reactive Oxygen Species / metabolism*
  • Skin / cytology*
  • Solutions
  • Ultraviolet Rays
  • tert-Butylhydroperoxide / pharmacology

Substances

  • Matrix Metalloproteinase Inhibitors
  • Plant Extracts
  • Reactive Oxygen Species
  • Solutions
  • tert-Butylhydroperoxide
  • Hydrogen Peroxide
  • Matrix Metalloproteinase 1