Rapid and highly sensitive neuraminidase subtyping of avian influenza viruses by use of a diagnostic DNA microarray

J Clin Microbiol. 2009 Sep;47(9):2985-8. doi: 10.1128/JCM.00850-09. Epub 2009 Jul 8.

Abstract

Rapid neuraminidase subtyping of avian influenza viruses from diagnostic samples is crucial considering the existence of permanently emerging and evolving strains. Here we report an easy-to-use, low-cost microarray for neuraminidase subtyping following fragment amplification by a generic, neuraminidase-specific reverse transcription-PCR (RT-PCR). This method enables highly specific characterization with a sensitivity equal to that of matrix gene-specific real-time RT-PCR.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Birds
  • Genotype
  • Humans
  • Influenza A virus / classification*
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza in Birds / diagnosis
  • Influenza in Birds / virology*
  • Influenza, Human / diagnosis
  • Influenza, Human / virology
  • Neuraminidase / genetics*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Viral Proteins / genetics*
  • Virology / methods*

Substances

  • Viral Proteins
  • NA protein, influenza A virus
  • Neuraminidase