Replete vitamin D stores predict reproductive success following in vitro fertilization

Fertil Steril. 2010 Sep;94(4):1314-1319. doi: 10.1016/j.fertnstert.2009.05.019. Epub 2009 Jul 8.


Objective: To determine whether 25OH-D levels in the follicular fluid (FF) of infertile women undergoing IVF demonstrate a relationship with IVF cycle parameters and outcome, hypothesizing that levels of 25OH-D in body fluids are reflective of vitamin repletion status.

Design: Prospective cohort study.

Setting: Academic tertiary care center.

Patient(s): Eighty-four infertile women undergoing IVF.

Intervention(s): Follicular fluid from follicles>or=14 mm; serum (n = 10) and FF levels of 25OH-D.

Main outcome measure(s): Clinical pregnancy (CP), defined as evidence of intrauterine gestation sac on ultrasound, following IVF; IVF cycle parameters.

Result(s): Serum and FF levels of 25OH-D were highly correlated (r=0.94). In a predominantly Caucasian population (66%), significantly lower FF 25OH-D levels were noted in Black versus non-Black patients. Significant inverse correlations were seen between FF 25OH-D levels and body mass index (r=-0.25). Significantly higher CP and implantation rates were observed across tertiles of FF25OH-D; patients achieving CP following IVF (n=26) exhibited significantly higher FF levels of 25OH-D. Multivariable logistic regression analysis confirmed FF 25OH-D levels as an independent predictor to success of an IVF cycle; adjusting for age, body mass index, ethnicity, and number of embryos transferred, each ng/mL increase in FF 25OH-D increased the likelihood for achieving CP by 6%.

Conclusion(s): Our findings that women with higher vitamin D level in their serum and FF are significantly more likely to achieve CP following IVF-embryo transfer are novel. A potential for benefit of vitamin D supplementation on treatment success in infertile patients undergoing IVF is suggested and merits further investigation.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Cohort Studies
  • Embryo Implantation / physiology
  • Female
  • Fertilization in Vitro*
  • Follicular Fluid / chemistry*
  • Follicular Fluid / metabolism
  • Humans
  • Infertility, Female / blood
  • Infertility, Female / diagnosis*
  • Infertility, Female / metabolism
  • Infertility, Female / therapy*
  • Predictive Value of Tests
  • Pregnancy
  • Pregnancy Rate
  • Reproduction / physiology
  • Treatment Outcome
  • Vitamin D / analysis*
  • Vitamin D / blood
  • Vitamin D / metabolism


  • Vitamin D