Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself

Clin Immunol. 2009 Oct;133(1):45-51. doi: 10.1016/j.clim.2009.06.004. Epub 2009 Jul 9.


EMT-6 mammary carcinoma and B16 melanoma (B16M) cells are lethal and barely immunogenic in syngeneic BALB/c and C57BL/6 mice, respectively. We show that mice vaccinated with tumor cells pulsed with a MHC class I-restricted peptide develop a T cell response, not only to the peptide, but also to the unpulsed tumor. These mice display protective immunity against the unpulsed tumor, and their T cells adoptively transfer tumor-specific protection to immunodeficient SCID mice. Our data have implications for cancer vaccine strategies. Grafting a single well-defined foreign peptide on tumor cells might suffice to trigger anti-tumor immunity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Cancer Vaccines / immunology*
  • Cell Line, Tumor
  • Histocompatibility Antigens Class I / immunology
  • Mammary Neoplasms, Animal / immunology*
  • Mammary Neoplasms, Animal / therapy
  • Melanoma, Experimental / immunology*
  • Melanoma, Experimental / therapy
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, SCID
  • Peptides / immunology*
  • Peptides / metabolism
  • Vaccination


  • Cancer Vaccines
  • Histocompatibility Antigens Class I
  • Peptides