Variation in the safety of induced pluripotent stem cell lines

Nat Biotechnol. 2009 Aug;27(8):743-5. doi: 10.1038/nbt.1554. Epub 2009 Jul 9.


We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Transformation, Neoplastic / pathology
  • Mice
  • Neurons / cytology
  • Pluripotent Stem Cells / cytology*
  • Safety*
  • Teratoma / pathology