Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation

Cell. 1991 Nov 29;67(5):917-26. doi: 10.1016/0092-8674(91)90365-6.

Abstract

In the Drosophila female the product of the germline stem cell, the cystoblast, gives rise to 16 interconnected cystocytes. One of them differentiates into the oocyte, while the 15 others become polyploid nurse cells. Bic-D is required for the differentiation of an oocyte and hence for fertility. Recessive mutations in Bic-D block the oocyte-specific accumulation of its own and other RNAs. Based on its properties and distribution, the Bic-D protein appears to be a component of a cytoskeletal transport or anchoring system. Additional results suggest that the phosphorylation of the Bic-D protein is essential for its accumulation in the pro-oocyte and that this process leads to the gradual localization to the pro-oocyte of factors required for oocyte differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Antibodies, Monoclonal
  • Cell Differentiation
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / physiology*
  • Female
  • Genes, Recessive
  • Genes, Suppressor
  • Immunoblotting
  • Insect Hormones / analysis
  • Insect Hormones / genetics
  • Insect Hormones / physiology*
  • Mutation
  • Oocytes / cytology*
  • Oocytes / physiology
  • Phosphorylation

Substances

  • Antibodies, Monoclonal
  • BicD protein, Drosophila
  • Drosophila Proteins
  • Insect Hormones