[MMP- and FAP-mediated non-inflammation-related destruction of cartilage and bone in rheumatoid arthritis]

Z Rheumatol. 2009 Oct;68(8):683-94. doi: 10.1007/s00393-009-0461-3.
[Article in German]

Abstract

Introduction: Due to morphological similarities of high-grade synovitis in rheumatoid Arthritis (RA) and mesenchymal, semimalignant tumors and the hypothesis that RA progression is not only inflammation-related, but also determined by tumor-like mechanisms, a comparison was made between expression profiles of RA, giant cell tumor of bone (GCT) and normal synovium (ND).

Methods: Array data of selected genes were validated through immunohistochemical staining of paraffin-embedded and deep frozen tissue samples of GCT, RA and normal synovium.

Results: With microarray analysis, CCR1, CCR5, MMP-1, MMP-2, MMP-3, MMP-9, MMP-14 and FAP were found to be significantly upregulated in RA and GCT compared to ND. A significant upregulation in RA and GCT compared to ND could be validated by immunohistochemistry for MMP-1, MMP-9, MMP-14 and FAP.

Discussion: For MMPs, and MMP-9 in particular, an important role in early cartilage destruction of RA was suggested. The presence of FAP in RA and in stroma of a semimalignant tumor indicates tumor-like tissue destruction in chronic synovitis associated with RA.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Aged
  • Arthritis, Rheumatoid / diagnosis
  • Arthritis, Rheumatoid / immunology*
  • Biomarkers / analysis
  • Bone and Bones / immunology*
  • Cartilage / immunology*
  • Female
  • Gelatinases / analysis*
  • Humans
  • Male
  • Matrix Metalloproteinases / analysis*
  • Membrane Proteins / analysis*
  • Receptors, CCR / analysis*
  • Serine Endopeptidases / analysis*

Substances

  • Biomarkers
  • Membrane Proteins
  • Receptors, CCR
  • Serine Endopeptidases
  • fibroblast activation protein alpha
  • Gelatinases
  • Matrix Metalloproteinases