Abstract
Human cystathionine beta-synthase (CBS) is a pyridoxal 5'-phosphate (PLP) dependent hemoprotein, which catalyzes the condensation of serine and homocysteine. Our mutagenesis studies suggest that Arg-266 is important to sense structural changes in heme-binding site, and that Gln-222 as well as Tyr-223 are involved in interactions with substrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Catalytic Domain
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Cystathionine beta-Synthase / chemistry*
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Cystathionine beta-Synthase / genetics
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Cystathionine beta-Synthase / metabolism*
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Heme / chemistry*
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Heme / genetics
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Heme / metabolism*
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Homocysteine / metabolism
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Humans
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Hydrogen Sulfide / metabolism
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Models, Molecular
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Mutagenesis, Site-Directed / methods*
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Protein Binding
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Serine / metabolism
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Spectrophotometry
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Structure-Activity Relationship
Substances
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Homocysteine
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Heme
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Serine
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Cystathionine beta-Synthase
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Hydrogen Sulfide