Establishment of a Germ-Line Competent C57BL/6 Embryonic Stem Cell Line

Exp Cell Res. 1991 Dec;197(2):254-8. doi: 10.1016/0014-4827(91)90430-3.

Abstract

Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Cell Line
  • Cell Separation / methods
  • Chimera
  • Chromosome Aberrations
  • Culture Techniques / methods
  • Embryo, Mammalian
  • Female
  • Genotype
  • Karyotyping
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mutation
  • Ploidies
  • Stem Cells / cytology*
  • Y Chromosome