Localization of mRNA for testis-specific histone H1t by in situ hybridization

Exp Cell Res. 1991 Dec;197(2):330-2. doi: 10.1016/0014-4827(91)90442-w.

Abstract

H1t is a testis-specific H1 histone variant that appears in germ cells during the meiotic prophase of mammalian spermatogenesis. Using a tritiated antisense RNA probe, H1t mRNA was identified by in situ hybridization in the mid and late pachytene spermatocytes found in seminiferous tubules of approximately stages VII to XIII.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antisense Elements (Genetics)
  • Cloning, Molecular
  • Histones / genetics*
  • Male
  • Meiosis
  • Nucleic Acid Hybridization
  • RNA Probes
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Rats
  • Rats, Inbred Strains
  • Restriction Mapping
  • Spermatocytes / cytology
  • Spermatocytes / physiology
  • Spermatogenesis
  • Testis / cytology
  • Testis / physiology*

Substances

  • Antisense Elements (Genetics)
  • Histones
  • RNA Probes
  • RNA, Messenger