The Formation of 3 Alpha- And 3 Beta-Acetoxytropanes by Datura Stramonium Transformed Root Cultures Involves Two acetyl-CoA-dependent Acyltransferases

FEBS Lett. 1991 Nov 4;292(1-2):293-7. doi: 10.1016/0014-5793(91)80887-9.


Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-ol) with acetyl-coenzyme A is also present. These two activities can be partially resolved by anion-exchange chromatography, some fractions containing only the pseudotropine-utilizing activity. The basic properties of these two enzymes are reported and their roles in forming the observed alkaloid spectrum of D. stramonium roots discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / isolation & purification
  • Acetyltransferases / metabolism*
  • Atropine / biosynthesis
  • Chromatography, Ion Exchange
  • Datura stramonium / metabolism*
  • Hydrogen-Ion Concentration
  • Plants, Medicinal*
  • Plants, Toxic*
  • Tropanes / metabolism*


  • Tropanes
  • 3-acetyltropine
  • Atropine
  • tropine
  • Acetyltransferases
  • acetyl CoA-tropine acetyltransferase
  • acetyl-CoA-pseudotropine acetyltransferase